FLSA: Salaried

Job Summary: The Mass Spectrometry Facility and Proteomics Center at the Medical University of South Carolina (MUSC) is recruiting a highly motivated post-doctoral associate to develop and apply LC-MS/MS-based proteomics methodology to elucidate disease mechanisms and response to therapeutics. These studies will include establishing targeted proteomic workflows for the quantitative measurement of peptide analytes/hormones that cannot be measured by antibody-based ELISAs and to develop biochemical methods to identify differentially and/or reciprocally regulated sites of Ser/Thr O-GlcNAcylation and phosphorylation. Experience in sample preparation, chromatography, nano-LC-MS/MS instrumentation, data acquisition, data analysis, and computational bioinformatic analysis of proteomics data is required. Instrumentation in the Proteomics Center includes a Thermo Scientific Orbitrap Fusion Lumos ETD/UVPD MS, Orbitrap Elite ETD MS, Waters Xevo TQ-S triple quadrupole MS for LC-MS/MS and a Bruker Tims-TOF Flex, Solarix 7T FT-ICR, and Rapiflex TOF-TOF for MALDI-imaging MS.

Qualifications:
• PhD in biochemistry, analytical chemistry, or related disciplines;
• Documented hands-on experience with quantitative LC-MS/MS;
• Experience with mapping sites of post-translational modifications;
• Ability to work accurately, with attention to detail, in a highly organized manner;
• Team player who can collaborate with clinicians and basic scientists;
• Excellent verbal and written communication skills.

Payscale Salary Range: UNIV-Band 0: Commensurate with experience.

Job Duties: 

• 25% - Establish targeted proteomic workflows for the quantitative measurement of peptide analytes and hormones in cells, tissues, or biofluids. - (Essential)
• 25% - Develop and optimize biochemical methods to identify differentially regulated sites of protein modifications. - (Essential)
• 25% - Perform proteomic experiments from start to finish including: experimental design, sample preparation, chromatography, nano-LC-MS/MS instrumentation, data acquisition, data analysis, computational bioinformatic analysis of proteomics data, and preparation of figures. - (Essential)
• 15% - Keep accurate records, prepare manuscripts and grant applications. - (Essential)
• 10% - Participate in Mass Spectrometry Journal club, training opportunities, and international conferences to communicate findings and keep up to date with technological advances. - (Essential)

Minimum Experience and Training Requirements: Ph.D.

Physical Requirements: Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32? F to 100? F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/20 vision, corrected. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous)